PLASMIDS

Our published plasmids are distributed by Addgene.


PROTOCOLS

J. D. Martell, T. J. Deerinck, S. S. Lam, M. H. Ellisman, and A. Y. Ting. Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells. Nature Protocols 2017, 12, 1792-1816. [PubMed][PDF]

V. Hung, N. D. Udeshi, S. S. Lam, K. H. Loh, K. J. Cox, K. Pedram, S. A. Carr, and A. Y. Ting. Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2. Nature Protocols 2016, 11, 456-475. [PubMed][PDF]

C. Uttamapinant, M. I. Sanchez, D. S. Liu, J. Z. Yao, K. A. White, S. Grecian, S. Clarke, K. R. Gee, and A. Y. Ting. Site-specific protein labeling using PRIME and chelation-assisted click chemistry. Nature Protocols 2013, 8, 1620-34. [PubMed][PDF]

M. Howarth and A. Y. Ting. Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin. Nature Protocols 2008, 3, 534-545. [PubMed][PDF]