PLASMIDS

Our published plasmids are distributed by Addgene.


PROTOCOLS

J. D. Martell, T. J. Deerinck, S. S. Lam, M. H. Ellisman, and A. Y. Ting. Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells. Nature Protocols 2017, 12, 1792-1816. [PubMed][PDF]

V. Hung, N. D. Udeshi, S. S. Lam, K. H. Loh, K. J. Cox, K. Pedram, S. A. Carr, and A. Y. Ting. Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2. Nature Protocols 2016, 11, 456-475. [PubMed][PDF]

C. Uttamapinant, M. I. Sanchez, D. S. Liu, J. Z. Yao, K. A. White, S. Grecian, S. Clarke, K. R. Gee, and A. Y. Ting. Site-specific protein labeling using PRIME and chelation-assisted click chemistry. Nature Protocols 2013, 8, 1620-34. [PubMed][PDF]

M. Howarth and A. Y. Ting. Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin. Nature Protocols 2008, 3, 534-545. [PubMed][PDF]


SOME PUBLICATIONS BY LABS USING APEX FOR PROTEOMICS

B. T. Lobingier et al. An approach to spatiotemporally resolve protein interaction networks in living cells. Cell 2017, 169, 350–360. [PubMed]

J. Paek et al. Multidimensional tracking of GPCR signaling via peroxidase-catalyzed proximity labeling. Cell 2017, 169, 338–349. [PubMed]

J. Hwang et al. A Golgi rhomboid protease Rbd2 recruits Cdc48 to cleave yeast SREBP. EMBO Journal 2016, 35, 2332-2349. [PubMed]

H. L. Wang et al. Dexamethasone-induced cellular tension requires a SGK1-stimulated Sec5-GEF-H1 interaction. J. Cell Science 2015, 128, 3757-3768. [PubMed]

J. Hwang and P. J. Espenshade. Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2. Biochemical Journal 2016, 473, 2463-2469. [PubMed]

D. U. Mick et al. Proteomics of primary cilia by proximity labeling. Developmental Cell 2015, 35, 497-512. [PubMed]

J. Jing et al. Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca(2+) influx. Nature Cell Biology 2015, 17, 1339-1347. [PubMed]

C. L. Chen et al. Proteomic mapping in live Drosophila tissues using an engineered ascorbate peroxidase. Proceedings of the National Academy of Sciences 2015, 112, 12093-12098. [PubMed]