PLASMIDS

Our published plasmids are distributed by Addgene.


PROTOCOLS

F. M. Fazal, S. Han, K. R. Parker, P. Kaewsapsak, J. Xu, A. N. Boettiger, H. Y. Chang*, and A. Y. Ting*. APEX-seq: RNA subcellular localization by proximity labeling. Protocol Exchange 2019. [APEX-seq protocol]

J. D. Martell, T. J. Deerinck, S. S. Lam, M. H. Ellisman, and A. Y. Ting. Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells. Nature Protocols 2017, 12, 1792-1816. [PubMed][PDF]

V. Hung, N. D. Udeshi, S. S. Lam, K. H. Loh, K. J. Cox, K. Pedram, S. A. Carr, and A. Y. Ting. Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2. Nature Protocols 2016, 11, 456-475. [PubMed][PDF]

C. Uttamapinant, M. I. Sanchez, D. S. Liu, J. Z. Yao, K. A. White, S. Grecian, S. Clarke, K. R. Gee, and A. Y. Ting. Site-specific protein labeling using PRIME and chelation-assisted click chemistry. Nature Protocols 2013, 8, 1620-34. [PubMed][PDF]

M. Howarth and A. Y. Ting. Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin. Nature Protocols 2008, 3, 534-545. [PubMed][PDF]