PLASMIDS

Our published plasmids are distributed by Addgene.


PROTOCOLS

J. D. Martell, T. J. Deerinck, S. S. Lam, M. H. Ellisman, and A. Y. Ting. Electron microscopy using the genetic tag APEX2 in cultured mammalian cells. Nature Protocols 2017, accepted.

V. Hung, N. D. Udeshi, S. S. Lam, K. H. Loh, K. J. Cox, K. Pedram, S. A. Carr, and A. Y. Ting. Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2. Nature Protocols 2016, 11, 456-475. [PubMed][PDF]

C. Uttamapinant, M. I. Sanchez, D. S. Liu, J. Z. Yao, K. A. White, S. Grecian, S. Clarke, K. R. Gee, and A. Y. Ting. Site-specific protein labeling using PRIME and chelation-assisted click chemistry. Nature Protocols 2013, 8, 1620-34. [PubMed][PDF]

M. Howarth and A. Y. Ting. Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin. Nature Protocols 2008, 3, 534-545. [PubMed][PDF]


SOME PUBLICATIONS BY LABS USING APEX FOR PROTEOMICS

B. T. Lobingier et al. An approach to spatiotemporally resolve protein interaction networks in living cells. Cell 2017, 169, 350–360. [PubMed]

J. Paek et al. Multidimensional tracking of GPCR signaling via peroxidase-catalyzed proximity labeling. Cell 2017, 169, 338–349. [PubMed]

J. Hwang et al. A Golgi rhomboid protease Rbd2 recruits Cdc48 to cleave yeast SREBP. EMBO Journal 2016, 35, 2332-2349. [PubMed]

A. W. Reinke, R. Mak, E. R. Troemel, and E. J. Bennett. In vivo mapping of tissue- and subcellular-specific proteomes in Caenorhabditis elegans. bioRxiv 2016 [preprint]

A. W. Reinke, K. M. Balla, E. J. Bennett, and E. R. Troemel. Identification of microsporidia host-exposed proteins reveals a repertoire of large paralogous gene families and rapidly evolving proteins. bioRxiv 2016 [preprint]

H. L. Wang et al. Dexamethasone-induced cellular tension requires a SGK1-stimulated Sec5-GEF-H1 interaction. J. Cell Science 2015, 128, 3757-3768. [PubMed]

J. Hwang and P. J. Espenshade. Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2. Biochemical Journal 2016, 473, 2463-2469. [PubMed]

D. U. Mick et al. Proteomics of primary cilia by proximity labeling. Developmental Cell 2015, 35, 497-512. [PubMed]

J. Jing et al. Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca(2+) influx. Nature Cell Biology 2015, 17, 1339-1347. [PubMed]

C. L. Chen et al. Proteomic mapping in live Drosophila tissues using an engineered ascorbate peroxidase. Proceedings of the National Academy of Sciences 2015, 112, 12093-12098. [PubMed]


SOME PUBLICATIONS BY LABS USING APEX FOR MICROSCOPY

J. P. Zumthor et al. Static clathrin assemblies at the peripheral vacuole—plasma membrane interface of the parasitic protozoan Giardia lamblia. PLoS Pathogens 2016, DOI:10.1371/journal.ppat.1005756. [PubMed]

J. C. D. Long and E. Fodor. The PB2 subunit of the influenza A virus RNA polymerase is imported into the mitochondrial matrix. Journal of Virology 2016, epub ahead of print. [PubMed]

A. Ludwig, B. J. Nichols, and S. Sandin. Architecture of the caveolar coat complex. Journal of Cell Science 2016, epub ahead of print. [PubMed]

C. D. Marceau et al. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 2016, 535, 159-163. [PubMed]

M. Joesch et al. Reconstruction of genetically identified neurons imaged by serial-section electron microscopy. eLife 2016, 10.7554/eLife.15015. [PubMed]

W. Heusermann et al. Exosomes surf on filopodia to enter cells at endocytic hot spots, traffic within endosomes, and are targeted to the ER. Journal of Cell Biology 2016, 213, 173–184. [PubMed]

N. S. Eyre et al. Phosphorylation of NS5A serine-235 is essential to hepatitis C virus RNA replication and normal replication compartment formation. Virology 2016, 491, 27-44. [PubMed]

U. L. Bohm et al. CSF-contacting neurons regulate locomotion by relaying mechanical stimuli to spinal circuits. Nature Communications 2016, 7, 10866. [PubMed]

T. Y. Lin et al. Mapping chromatic pathways in the Drosophila visual system. J. Comparative Neurology 2016, 524, 213-227. [PubMed]

X. Luo et al. Enhanced transcriptional activity and mitochondrial localization of STAT3 co-induce axon regrowth in the adult central nervous system. Cell Reports 2016, 15, 398-410. [PubMed]

N. Ariotti et al. Modular detection of GFP-labeled proteins for rapid screening by electron microscopy in cells and organisms. Developmental Cell 2015, 35, 513-525. [PubMed]

J. Fueller et al. Subcellular partitioning of protein tyrosine phosphatase 1B to the endoplasmic reticulum and mitochondria depends sensitively on the composition of its tail anchor. PLoS ONE 2015, 10, e0139429. [PubMed]

V. Hyenne et al. RAL-1 controls multivesicular body biogenesis and exosome secretion. Journal of Cell Biology 2015, 211, 27-37. [PubMed]

J. Lee et al. An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier. Chemical Communications 2015, 51, 10945-10948. [PubMed]

G. Bertolin et al. Parkin maintains mitochondrial levels of the protective Parkinson's disease-related enzyme 17-β hydroxysteroid dehydrogenase type 10. Cell Death and Differentiation 2015, 22, 1563-1576. [PubMed]

E. Shvets, V. Bitsikas, G. Howard, C. G. Hansen, and B. J. Nichols. Dynamic caveolae exclude bulk membrane proteins and are required for sorting of excess glycosphingolipids. Nature Communications 2015, 6, 6867. [PubMed]

A. D. Lawrence et al. Solution structure of a bacterial microcompartment targeting peptide and its application in the construction of an ethanol bioreactor. ACS Synthetic Biology 2014, 3, 454-465. [PubMed]

M. Wong and S. Munro. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 2014, 346, 1256898. [PubMed]